A Chlamydomonas outer arm dynein mutant missing the alpha heavy chain.

A Chlamydomonas outer arm dynein mutant missing the alpha heavy chain

A novel Chlamydomonas flagellar mutant (oda-11) missing the alpha heavy chain of outer arm dynein but retaining the beta and gamma heavy chains was isolated. Restriction fragment length polymorphism analysis with an alpha heavy chain locus genomic probe indicated that the oda-11 mutation was genetically linked with the structural gene of the alpha heavy chain. In cross-section electron microgra...

A Chlamydomonas Outer Arm Dynein Mutant Missing the te Heavy Chain

A novel Chlamydomonas flagellar mutant (oda-11) missing the ot heavy chain of outer arm dynein but retaining the/3 and 3~ heavy chains was isolated. Restriction fragment length polymorphism analysis with an ct heavy chain locus genomic probe indicated that the oda-11 mutation was genetically linked with the structural gene of the tx heavy chain. In crosssection electron micrographs, the oda-ll ...

A Chlamydomonas Outer Arm Dynein Mutant with a Truncated Heavy Chain

A new allele of the Chlamydomonas oda4 flagellar mutant (oda4-s7) possessing abnormal outer dynein arms was isolated. Unlike the previously described oda4 axoneme lacking all three (a, /3, and 3") outer-arm dynein heavy chains, the oda4-s7 axoneme contains the c~ and 3' heavy chains and a novel peptide with a molecular mass of •160 kD. The peptide reacts with a mAb (18~B) that recognizes an epi...

Partially Functional Outer-Arm Dynein in a Novel Chlamydomonas Mutant Expressing a Truncated Heavy Chain

A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain

A new allele of the Chlamydomonas oda4 flagellar mutant (oda4-s7) possessing abnormal outer dynein arms was isolated. Unlike the previously described oda4 axoneme lacking all three (alpha, beta, and gamma) outer-arm dynein heavy chains, the oda4-s7 axoneme contains the alpha and gamma heavy chains and a novel peptide with a molecular mass of approximately 160 kD. The peptide reacts with a mAb (...